Bartosz Kempisty PhD.,

Associate Professor Department of Histology and Embryology, Department of Anatomy Poznan University of Medical Sciences, Poznan, Poland, Department of Veterinary Surgery, Institute of Veterinary Medicine Nicolaus Copernicus University, Torun, Poland, Visiting Professor Center of Assisted Reproduction-Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Brno, Czech Republic, Editor-in-chief Medical Journal of Cell Biology.

My research team consists of several members; three associate professors, one post-doc, as well as a group of PhD students. Other affiliates include clinicians, biotechnologists, bioengineers, as well as biologists such as: histologists, embryologists and human anatomists.

In the years 2008-2019, our team conducted and currently conducts 19 research projects financed by Polish National Science Centre and two financed by European Union funds. My team collaborates with many institutions in the country, Europe, as well as rest of the world, such as: King’s College (London, UK), Leuven University (Belgium), Institute for Farm Animals Biology (Dummerstorf, Germany), University of Veterinary Medicine (Budapest, Hungary), Veterinary Research Institute (Brno, Czech Republic), Masaryk University (Brno, Czech Republic), College of Food & Agriculture Sciences (King Saud University, Saudi Arabia), University of Tel Aviv (Israel), North Carolina State University (USA), Children’s Medical center Hospital (Dr. Qarib St, Keshavarz Blvd, Teheran 14194, Iran), Faculty of Science (University of Technology Sydney).

My main research topics include: • Stemness properties of primary cultured reproductive cells and tissues (based on human and animal model-see attachment with table and types of cells banked) • Stemness properties and capacity of oral-derived cells (mostly fibroblasts and keratinocytes), (human gingiva, human dental pulp stem cells, porcine buccal pouch mucosal and fascial cells). • Evaluation of physiological properties of HUVECs and Wharton Jelly stem cells and their possible application in regenerative and reconstructive medicine and veterinary practice • Stemness properties of ADSCs used as the model for psychiatric drug mediation, metabolism and action • Human and animal model based on ADSCs • Cardio-vascular projects based on stemness competence of cardiac cells and their proangiogenic properties (human and animal – pig model) • Research based on transcriptome of human spermatozoa collected from normospermic, azoospermic and asthenospermic patients Etc., Methods used in routine Lab work: • Primary cell culture (short-term, long-term) • RTCA (real-time cells proliferation analysis) • Primary monolayer and 3D culture models • Paracrine activity of cells (culture inserts) • Identification of cellular ECM • RNA Microarrays • RT-qPCR • Flow cytometry • MALDI-TOF LCMS/MS • Western blot • ELISA • Histochemistry • Immunohistochemistry • Tissue microarrays • Light, fluorescence and confocal microscopy

Co-author of 254 scientific publications (IF>300, H-index=20) in the field of cellular and molecular biology and bioengineering. In his scientific work, his main current focus is the biology and biotechnology of stem cells obtained from many organs and tissues, such as; human and animal ovarian granulosa and cumulus oophorus cells, luminal epithelial endometrial cells, oviductal epithelial cells, HUVECs, Wharton Jelly, human cytotrophoblast cells, dental pulp cells, gingival cells and human oral mucosa, human vascular endothelium, human progenitor cardiac cells (cardiomyoblasts), human vascular stem/progenitor cells, and many others. In addition, he conducts studies using animal models for the analysis of the regenerative capacity of the tissues. He cooperates with many prestigious universities locally and abroad. The main research methods that he implements in the laboratory are; primary 2D and 3D insert cultures, flow cytometry, RNA and miRNA microarrays, LCMS / MS. The advanced transcriptomic and proteomic tests that he uses result in the identification of new markers of processes related to the development, proliferation and differentiation of stem cells in in vitro primary culture models. Main medical research area: • Reproduction (gynecology and obstetrics) • Reproductive stem cells • Experimental cardiology • Vascular surgery • Medicine based on stem cells • Dentistry and oral biology • Stem cells transplantation • Translational medicine